Proteomics analysis of Echinococcus granulosus protoscolex stage.
نویسندگان
چکیده
Echinococcus granulosus protoscolex proteins were separated using two-dimensional electrophoresis and then identified using mass spectrometry; we identified 61 proteins, 28 which are newly described of which 4 could be involved in hydatid cyst fertility molecular mechanisms.
منابع مشابه
Evaluation the scolicidal and cytotoxic effects of Froriepia subpinnataagainst protoscolex of Echinococcus granulosus in vitro
Background & Aim: Hydatidosis is one of the most important and prevalent parasitic diseases in Iran. Due to the fact that no definite and reliable treatment for this disease has been recorded so far, the aim of this study was to investigate the antiparasitic effect of Froriepia subpinnata (F. subpinnata) on the protoscolex stage of Echinococcus granulosus (E. granulosus) in vitro. Methods: F. ...
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Echinococcosis caused by the larval form of Echinococcus granulosus (E. granulosus) is known as an important zoonotic disease in various parts of the world, including Iran. The genetic diversity of this parasite is very high, particularly in areas where the disease is endemic. It has been suggested in the literature from different parts of the world that diverse facto...
متن کاملIdentification, expression and phylogenetic analysis of EgG1Y162 from Echinococcus granulosus.
OBJECTIVE This study was to clone, identify and analyze the characteristics of egG1Y162 gene from Echinococcus granulosus. METHODS Genomic DNA and total RNAs were extracted from four different developmental stages of protoscolex, germinal layer, adult and egg of Echinococcus granulosus, respectively. Fluorescent quantitative PCR was used for analyzing the expression of egG1Y162 gene. Prokaryo...
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OBJECTIVES Infection of humans and domestic ruminants with the larval stage (metacestode) of Echinococcus granulosus results in cystic echinococcosis (CE). The metacestode causes a space-occupying lesion in visceral organs, most commonly in the liver. Benzimidazole carbamate derivatives, such as mebendazole and albendazole, are currently used for chemotherapeutic treatment of CE. In human patie...
متن کاملTrials to induce protective immunity in mice and sheep by application of protoscolex and hydatid fluid antigen or whole body antigen of Echinococcus granulosus.
In the current study, soluble proteins prepared from 200 mature Echinococcus granulosus and protoscolices of sheep hydatid cysts were applied to immunize sheep and mice respectively. The samples were mechanically homogenized in a blender, sonicated and the final yield was maintained at -20 degrees C until analysis. Hydatid fluid was isolated from liver or lung of sheep under sterile conditions....
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ورودعنوان ژورنال:
- Veterinary parasitology
دوره 218 شماره
صفحات -
تاریخ انتشار 2016